Journal: Nutrients

Article Title: Multifaceted Protective Effects of Hesperidin by Aromatic Hydrocarbon Receptor in Endothelial Cell Injury Induced by Benzo[a]Pyrene

doi: 10.3390/nu14030574

Figure Lengend Snippet: Effect of Hesperidin in mediating low density lipoprotein (LDL) accumulation in EA.hy926 induced by benzo[a]pyrene (BaP). ( A ) Representative immunocytochemistry of aromatic hydrocarbon receptor (AHR) level in EA.hy926 ( n = 3 per group, Scale bars, 100 μm). ( B ) mRNA levels of AHR in EA.hy926 ( n = 3 per group). * p < 0.05, vs. normal control group, # p < 0.05, vs. BaP control group. ( C ) mRNA levels of cytochrome P450 1A1 (CYP1A1) in EA.hy926 ( n = 3 per group). ** p < 0.01 vs. normal control group, ## p < 0.01 vs. BaP control group. ( D ) mRNA levels of CYP1A1 in EA.hy926 ( n = 3 per group). ** p < 0.01 vs. LDL control group, ## p < 0.01 vs. LDL + BaP group. ( E ) The uptake of FITC-LDL in EA.hy926 was observed with laser confocal microscopy ( n = 3 per group). ( F ) Representative immunocytochemistry of Adenosine Triphosphate-binding cassette transporter A1 (ABCA1) level in EA.hy926. ( n = 3 per group, Scale bars, 100 μm). ( G ) mRNA levels of ABC1A1 in EA.hy926 ( n = 3 per group) * p < 0.05, vs. normal control group, ## p < 0.01 vs. BaP control group. ( H ) Representative immunocytochemistry of advanced glycation end products (AGEs) level in EA.hy926. ( n = 3 per group, Scale bars, 100 μm). The data are presented as means ± SD ( n = 3) and were analyzed using one-way ANOVA. * p < 0.05, ** p < 0.01 vs. normal control group, # p < 0.05, ## p < 0.01 vs. BaP control group.

Article Snippet: For immunohistochemistry experiments, the cells were incubated with anti-AHR mouse monoclonal primary antibodies (1:200, SC133088, Santa Cruz, CA, USA), anti-ABCA1 mouse monoclonal primary antibodies (1:200, SC53482, Santa Cruz, CA, USA), anti-AGEs rabbit polyclonal primary antibodies (1:200, bs-1158R, BIOSS, Beijing, China), and anti-TNF-α rabbit polyclonal primary antibodies (1: 200, GTX110520, Gentex, MI, USA) overnight at 4 °C.

Techniques: Immunocytochemistry, Control, Confocal Microscopy, Binding Assay

Journal: Nutrients

Article Title: Multifaceted Protective Effects of Hesperidin by Aromatic Hydrocarbon Receptor in Endothelial Cell Injury Induced by Benzo[a]Pyrene

doi: 10.3390/nu14030574

Figure Lengend Snippet: Primers for real-time qPCR.

Article Snippet: For immunohistochemistry experiments, the cells were incubated with anti-AHR mouse monoclonal primary antibodies (1:200, SC133088, Santa Cruz, CA, USA), anti-ABCA1 mouse monoclonal primary antibodies (1:200, SC53482, Santa Cruz, CA, USA), anti-AGEs rabbit polyclonal primary antibodies (1:200, bs-1158R, BIOSS, Beijing, China), and anti-TNF-α rabbit polyclonal primary antibodies (1: 200, GTX110520, Gentex, MI, USA) overnight at 4 °C.

Techniques: Sequencing

Journal: Foods

Article Title: The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion

doi: 10.3390/foods10112852

Figure Lengend Snippet: The primer sequences for qRT-PCR analysis.

Article Snippet: Mouse monoclonal Anti-ABCA1 antibody , Abcam , ab18180.

Techniques:

Journal: Foods

Article Title: The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion

doi: 10.3390/foods10112852

Figure Lengend Snippet: Antibodies used for Western blot.

Article Snippet: Mouse monoclonal Anti-ABCA1 antibody , Abcam , ab18180.

Techniques: Western Blot